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Fig. 1 | Biology Direct

Fig. 1

From: Dynamic expression of IGFBP3 modulate dual actions of mineralization micro-environment during tooth development via Wnt/beta-catenin signaling pathway

Fig. 1

The spatiotemporal expression pattern of IGFBP3. A Volcano plot (a, ME vs. TG) and heatmap (b) demonstrating extracellular protein level difference introduced by dental epithelium–mesenchyme dissociation. B Isolation of tooth germ tissue from E14.5 mouse embryo mandibles and subsequent dissociation study (a). RT-qPCR analysis compared Igfbp3 levels in each group (b). C Tooth germs of first molars were isolated from E14.5-P9 mandibles (a). The mRNA levels of Igfbp3 (b) and osteogenic markers Bmp2 (c1), Ocn (c2), Opn (c3), Osx (c4) and Alp (c5) in tooth germs from E14.5 to P9 were detected by RT-qPCR. D Immunohistochemical analyses were performed to evaluate the expression of IGFBP3 at different stages of tooth development. ME dental mesenchyme alone, TG whole tooth germ, TG-ME dental mesenchyme isolated after organ culture; Bmp2, bone morphogenetic protein 2; Ocn, osteocalcin; Opn, osteopontin; Osx, osterix; Alp, alkaline phosphatase; ab, alveolar bone; am, ameloblasts; d, dentin; e, enamel; dm, dental mesenchyme; od, odontoblast. n = 3, *p < 0.05, **p < 0.01, ns: no significance. Scale bars: 750 μm (C-a), 50 μm (D)

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