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Fig. 5 | Biology Direct

Fig. 5

From: Single-cell transcriptomics reveals cellular heterogeneity and macrophage-to-mesenchymal transition in bicuspid calcific aortic valve disease

Fig. 5

Trajectory analysis identified MMT in bicuspid CAVD. (a) a re-analysis of previously published CAVD dataset. (b) Subclusters of monocytic cells in previous dataset. (c) Examine selected genes in different subclusters of monocytic cells. Data indicated that MDSC subpopulation was not identified in previous CAVD dataset. (d) Velocity graph of monocytic cells based on UMAP projection. The arrows on the graph indicates the future states of the cells inferred by RNA velocity. (e) Velocity pseudotime plot. (f) PAGA graph based on RNA velocity. Lines with arrows indicate high probability of transition. (g) Pseudotime trajectory computed by Monocle2. Trajectory showing 3 major branches (1 pre-branch and 2 cell fates). (h) Branched heatmap showing gene expressions change as pseudotime goes. DEGs was clustered into 3 groups. (i) The group of MMT genes was used to conduct gene ontology (GO) enrichment. ECM organization and ossification processes were enriched. (j) The expression dynamics of representative genes in branched cell fates

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