Fig. 4
From: PSMD3-ILF3 signaling cascade drives lung cancer cell proliferation and migration
PSMD3 binds to ILF3. (A) Venn diagram of common genes using RNA pull-down assay and mass spectrometry for identification of PSMD3-associated proteins with A549 cell lysates. (B) Co-IP was made with the ILF3 antibody in LC cells lentivirally transduced with the empty vector or vector encoding PSMD3-flag. WB was adopted to analyze immunoprecipitants. (C) Immunofluorescence staining for PSMD3-flag (red) and ILF3 (green) in LC cells. (D) The protein levels of ILF3 in LC cells with PSMD3 knockdown or overexpression. (E) Reduction of ILF3 protein stability in A549 cells with PSMD3 knockdown and in H1299 cells with PSMD3 overexpression. (F) The poly-ubiquitination of ILF3 in PSMD3 overexpression or knockdown cells were assessed using the ubiquitination assay. (G) The expression of PSMD3 and ILF3 in LC clinical tissues were analyzed using IHC staining. * p < 0.05, ** p < 0.01, *** p < 0.001