Table 3 List of the primary antibodies used in this study
Antibody | Catalogue number | Brand | Dilution | MW (kDa) | Application |
|---|---|---|---|---|---|
Iba1 | A20844 | Abclonal | 1:100 | NA | IF |
MAP2 | sc-74,421 | Santa Cruz | 1:100 | NA | IF |
NeuN | Ab177487 | abcam | 1:500 | NA | IF |
iNOS | 13,120 | CST | 1:200 | NA | IF |
NLRP3 | MAB7578 | R&D system | 12.5ug/ml | NA | IF |
NLRP3 | ab263899 | abcam | 1:1000 | 118 | WB |
Cleaved Caspase1 | 67,314 | CST | 1:1000 | 20/22 | WB |
GSDMD | Ab209845 | abcam | 1:1000 | 32/53 | WB |
ASC | 67,824 | CST | 1:1000 | 22 | WB |
CD63 | ab134045 | abcam | 1:1000 | 30–65 | WB |
Alix | 2171 | CST | 1:1000 | 95 | WB |
TSG101 | 72,312 | CST | 1:1000 | 50 | WB |
HIF1α | 14,179 | CST | 1:1000 | 120 | WB |
p-tau | 9632 | CST | 1:1000 | 50–80 | WB |
p-tau | 49,561 | CST | 1:200 | NA | IF |
β-actin | 4970 | CST | 1:1000 | 45 | WB |