Table 2 Primer sequences of miRNA and mRNAs oligomers for RT-PCR.
Gene | Primer sequence, 5’-3’ | |
|---|---|---|
Forward | Reverse | |
miR-146a-5p | TAATCGTGTGAGAACTGAATTCCA | TATGGTTTTGACGACTGTGTGAT |
U6 | CAGCACATATACTAAAATTGGAACG | ACGAATTTGCGTGTCATCC |
NLRP3 | GCCGTCTACGTCTTCTTCCTTTCC | CATCCGCAGCCAGTGAACAGAG |
IL-18 | AGACCTGGAATCAGACAACTTT | TCAGTCATATCCTCGAACACAG |
HIF1α | TTTCTCAGTCGACACAGCCT | AATTGAGCGGCCCAAAAGTT |
IL-1β | ATGGGCTGGACTGTTTCTAATGC | TCTTGTGACCCTGAGCGACC |
TNFα | CGGCATGGATCTCAAAGACAAC | GAAGAGAACCTGGGAGTAGACAAG |
GAPDH | GCCAAGGCTGTGGGCAAGGT | TCTCCAGGCGGCACGCAGA |