Fig. 5

Overexpressed miR-146a-5p in microglial exosomes alleviates neuronal NLRP3 inflammasome and neuroinflammation after IH. (a) Primary neurons were identified by transmission light microscope and IF staining of MAP2. Scale bar: 50 μm. (b) Immunofluorescence staining showed that PKH26 was co- expression with MAP2, demonstrating that exogenous exosomes were taken up by neurons. Scale bar: 50 μm. (c, d) Western blot (c) and quantitative data (d) of NLRP3 inflammasome proteins (NLRP3, Cleaved caspase1, ASC) in primary neurons after IH and treatment with exosomes. Data are presented as mean ± SD and analyzed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001