Fig. 4

The production of inflammatory cytokine in neuron is mediated by NLRP3 inflammasome. (a, c) NLRP3 and cleaved caspase1 protein were measured by immunoblot after 12 h of IH in HT22 hippocampal neurons. (b, d) Quantitative data of NLRP3 and cleaved caspase1 protein levels after 12 h of IH in hippocampal neurons. (e) The expression analysis of NLRP3 mRNA by qRT-PCR was significantly decreased following siNLRP3 transfection. (f) The hippocampal neurons cultured with the caspase-1 inhibitor Ac-YVAD-cmk (YVAD) or transfected with siNLRP3 and then treated with IH for 12 h. The cleaved caspase1 protein were performed by western blotting. (g) Quantitative analysis of cleaved caspase1 in neurons. (h) The mRNA expression levels of IL-1β, IL-18 was detected under the above different treatment. Data are presented as mean ± SD and analyzed using student’s t test or one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001. siNLRP3, a specific silencing RNA against NLRP3.