Fig. 3

Analysis of miR-146a-5p levels in microglia and microglial exosomes after IH12h. (a) Venn diagram depicting miRNAs related to inflammation and cognition. (b) The time changes of miR-146a-5p levels in microglial exosomes acquired from mice after IH determined by qRT-PCR. (c) Identification of BV2 microglia using transmission light microscope and immunofluorescence staining of Iba1. Scale bar: 50 μm. (d-f) Identification of microglial exosomes by nanoparticle tracking analyzer, immunoblot analysis (CD63, Alix and TSG101) and transmission electron microscopy scanning. Scale bar: 200 nm. (g) The level of miR-146a-5p in cultured microglia after miR-124-3p mimics transfection. (h) IF staining showed that the number of iNOS+ /DAPI + cells in miR-124-3p mimics + IH12h group was lower than that in the IH12h + Negative control (NC) group. Scale bar: 50 μm. Data are expressed as mean ± SD and analyzed using student’s t test or one-way ANOVA with Dunnett’s T3 post hoc test. *P < 0.05, **P < 0.01