Fig. 2

IH8w promoted microglial activation and increased p-tau expression in mice. (a) Representative immunofluorescence staining of Iba1 positive cells in the cortex and hippocampus of mice exposed 8 weeks to IH or normoxia. Scale bar: 50 μm. (b) Quantitative analysis of Iba1⁺ cells. (c) Endpoints of microglia were counted by Image J. (d) Total branch length were measured by Image J. (e) Representative images of p-tau and DAPI staining in the DG area of hippocampus of mice with IH for 8 weeks or normoxia. Scale bar: 100 μm. (f) The p-tau expression was significantly increased in the hippocampus of mice after IH8w. Data are expressed as mean ± SD and analyzed using student’s t test. *P < 0.05, **P < 0.01