Fig. 4

XIST promoted the pathological progression of HLF cells by activating autophagy. A The expression levels of autophagy-related proteins (LC3B, Beclin1, and p62) in LF tissues from patients with or without HLF were evaluated by western blotting. B The expression levels of autophagy-related protein (LC3B, Beclin1, and p62) in HLF cells infected with the indicated adenovirus were evaluated by western blotting. C The growth curves of HLF cells treated with the indicated adenovirus or 3-MA was evaluated by CCK-8 assays. D and E The apoptosis ratios of HLF cells treated with the indicated adenovirus or 3-MA were assessed by flow cytometry with Annexin V/PI double staining. F The protein expression of apoptosis-related genes (cleaved caspase3, Bax, and Bcl-2) in HLF cells treated with the indicated adenovirus or 3-MA was measured by western blotting. G The protein expression of fibrosis-related genes (collagen I, collagen III, MMP2, and MMP13) in HLF cells treated with indicated adenovirus or 3-MA was confirmed by western blotting. C-casp3, cleaved-caspase-3; shNC, corresponding negative control of shXIST; shXIST, the RNAi adenovirus against XIST; OE-NC, corresponding negative control of OE-XIST; OE-XIST, the overexpression adenovirus of XIST; 3-MA, autophagy inhibitor 3-methyladenine. ^*P < 0.05, ^**P < 0.01, and.^***P < 0.001