Fig. 8
ATM/p53 signaling pathway is involved in Ti3C2 nanosheets exposure-induced spermatogenesis disorder. a Volcano plot of DEGs. b Heat map of DEGs in the samples. c KEGG pathway enrichment analysis of gene expression in testes. d–g Testicular ATM, p-ATM, CHK2, p-CHK2, p53, p-p53 were detected using Western blotting. h–k Cellular ATM, p-ATM, CHK2, p-CHK2, p53, p-p53 were detected using Western blotting. All data were expressed as means ± standard deviation (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, compared with the control group