Fig. 5
Ti3C2 nanosheets exposure inhibits proliferation in mouse testes and GC-1 cells. a, b Cell proliferation was examined by using Ki67 staining. Ki67+ cells were counted. Magnification at 100 × and 400 ×. c, d Testicular PCNA was detected using Western blotting. e, f Cellular PCNA was detected using Western blotting. g The viability of GC-1 cells was examined via the using CCK-F assay. h GC-1 cells were stained with Ki67 by immunofluorescence. (γ-H2AX, DAPI, Merge, Magnification at 100 ×). All data were expressed as means ± standard deviation (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, compared with the control group