Fig. 4
Ti3C2 nanosheets exposure causes DNA damage in mouse testes and GC-1 cells. a DNA damage was shown via staining of the seminiferous tubules with the DSB marker γ-H2AX (red). Magnification at 400 ×. b, c Testicular γ-H2AX was detected using Western blotting. d, e The γ-H2AX protein level in GC-1 cells were detected using Western blotting. f The typical comet tails of GC-1 cells at different doses. Magnification at 200 ×. g Representative images of γ-H2AX (red puncta) detected by immunofluorescence (γ-H2AX, DAPI, Merge, Magnification at 100 ×). All data were expressed as means ± standard deviation (n = 3). *P < 0.05, **P < 0.01, compared with the control group