Fig. 9

Complementation of the disrupted mlf gene by transfection of MLF expression plasmid recovered cell growth after stress. A, B, C, D, E To complement the disrupted mlf gene, a pPMLF expression vector was transfected to the MLF KD and control cell lines. The complement and control cell lines were subcultured in growth medium containing (A) 80 μM MG132, (B) 36 μM rapamycin, (C) 100 μM chloroquine, (D) 5 μM nocodazole, and (E) 5 mM DTT for 24 h and then subjected to cell count. An equal volume of solvent (H2O or Me2SO) was added to cultures as an untreated control. Fold changes in cell number are shown as the ratio of cell number in the treatment relative to the control group. Values are shown as mean ± 95% confidence intervals of three independent experiments. *, p < 0.05. **, p < 0.01