Fig. 8

Effects of Bor on the frequency of immune cells and on the functional status of T cells recruited to the tumor microenvironment. Reported are mean ± SD values. Statistical significance of the differences observed between Bor-and vehicle-treated mice was evaluated by Student t-test (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). a Frequency of CD4⁺ and CD8⁺ T lymphocytes, B lymphocytes, Myeloid-derived suppressor cells (MDSCs) and Tumor associated macrophages (TAMs) in the spleen (SPL) and peritoneal ascitic fluid (ASC) of tumor-bearing mice (day 30) treated with Bor (n = 5) or vehicle (CTR) (n = 5), as assessed by flow cytometry. b Frequency of CD4⁺/CD69⁺, CD8⁺/CD69⁺ and of CD4⁺/CD25⁺, CD8⁺/CD25⁺ T lymphocytes in the spleen (SPL) or in the peritoneal ascitic fluid (ASC) of tumor-bearing mice (day 30) treated with Bor (n = 5) or with vehicle (CTR) (n = 5), as assessed by flow cytometry. c Frequency of CD4⁺/PD-1⁺, CD8⁺/PD-1⁺ and of CD4⁺/IFN-γ⁺, CD8⁺/IFN-γ⁺ T lymphocytes in the spleen (SPL) or in the peritoneal ascitic fluid (ASC) of tumor-bearing mice (day 30) treated with Bor (n = 5) or with vehicle (CTR) (n = 5), as assessed by flow cytometry