Fig. 3

WGCNA reveals different levels of synaptic organisation: pure gradients within cell types, intermediate gradients and discrete expression in specific cell types and cell classes. A WGCNA dendrogram and gene modules selected. B Density distribution of the eigenvector (PC1) that explains the variance of gene expression across cell types in the gene modules (80.3% variance explained). Colour coding corresponds to the K-means gene module classification according to their gradients across cell types. C Anticorrelation of the average gene expression of the turquoise and yellow gradient modules. D, F, H Sunburst plots showing the biological functions (left) and cellular components (right) for which the gene modules of each type show enrichment. Dark grey indicates non-significant SynGO terms that contain genes in the modules, and coloured SynGO terms indicate enrichment in one of the gene modules. E, G, I tSNE plot of example modules within each group colour coded with the average gene expression of the genes in each module