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Fig. 5 | Biology Direct

Fig. 5

From: USP5 knockdown alleviates lung cancer progression via activating PARP1-mediated mTOR signaling pathway

Fig. 5

USP5 promotes the progression of lung cancer by regulating PARP1. (A) USP5-Flag and PARP1-Flag were overexpressed in HEK293 cells. The cell lysates were subjected to immunoprecipitation with IgG and Flag and immunoblotting analysis of USP5 and PARP1. (B) USP5 and PARP1 protein levels were detected by immunoblotting in USP5 overexpressed and knockdown cells. (C) siCtrl and siUSP5 cells were treated with cycloheximide and subjected to immunoblotting analysis of USP5. (D) Ubiquitin was overexpressing in siCtrl and siUSP5 cells which were treated with MG132. The cells were IP with PARP1 antibody. Ubiquitionation abundance was detected by immunoblotting

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Biology Direct

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