Fig. 3
From: Image-based modeling of vascular organization to evaluate anti-angiogenic therapy
Comparison of selected intravital stained tumors with healthy organs. a Segmentation of the perfused vasculature in three different tumors. Detail showing the differences in surface structure of the vessels. White arrowheads: areas void of perfused vessels. Blue arrowheads: enlarged vessels. Yellow arrowheads: small capillaries. Green arrowheads: irregular protrusion on vessels in 4T1 tumors. Grid scale: 50 µm, SB: 100 µm. b Tracing results of perfused vessels in the respective tumor models. Vessel segments are displayed color-coded according to average diameters. Grid scale: 50 µm. c Heatmaps displaying distances from nearest perfused vessel in the respective tumors. SB: 100 µm. d Distance frequency distribution to closest perfused vessel in healthy organs. n ≥ 4. e Distance frequency distribution to closest perfused vessel in tumors. n ≥ 4. f Graphical explanation of the RM10 value on the example of the distance frequency distribution in the brain. g Comparison of RM10 values of normal organs and tumors. n ≥ 4. h Comparison of perfused vessel volume as fraction from total tissue volume of normal organs and tumors. n ≥ 4. i Results from PCA of vascular parameters in normal organs and in the three tumor models. Orientation of four highly ranked selected variables (blue arrows) are shown. Error bars: ± SEM. Asterisks display results from statistical tests with *: P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001