Fig. 1
From: Image-based modeling of vascular organization to evaluate anti-angiogenic therapy
Vascular structures in healthy organs. a Representative 3D-projection of the perfused vasculature in the frontal cortex of the murine brain after segmentation. SB: 100 µm. Insert: full representation of the tissue fragment acquired by LSFM. Grid scale: 50 µm. b Representative 3D-projection of the perfused vasculature in the murine skeletal muscle after segmentation. SB: 100 µm. Insert: full representation of the tissue fragment acquired by LSFM. Grid scale: 50 µm. c Tracing results of perfused vessels in the murine brain. Vessel segments are displayed color-coded according to average diameters. Grid scale: 50 µm; Color scale: Average vessel segment diameter: 2-15 µm. d Tracing results of perfused vessels in the murine skeletal muscle. Vessel segments are displayed color-coded according to average diameters. Grid scale: 50 µm; Color scale: Average vessel segment diameter: 2-15 µm. e Rendering of segmented vasculature in the murine brain. The majority of vessels in the displayed cubic section forms an interconnected structure (red). On the surfaces of the displayed structure are few vessel segments (purple) observable that are not connected within the visible volume to the bulk of vessels. Grid scale: 50 µm. f Rendering of segmented vasculature in the murine skeletal muscle. The majority of vessels in the displayed cubic section forms an interconnected structure (red). On the surfaces of the displayed structure several large vessel segments (purple) are observable that are not connected within the visible volume to the bulk of vessels. Grid scale: 50 µm. g Heatmap display of distances from nearest perfused vessel in the murine brain. SB: 100 µm. h Heatmap display of distances from nearest perfused vessel in the murine skeletal muscle. SB: 100 µm