Fig. 5

miR-657 targets DKK2 mRNA and DKK2 expression is positively regulated by LINC00326. a Schematic view of the miR-657 binding site in the wt and mut 3′-UTR of DKK2 mRNA. b DKK2 levels in tumor and normal tissues, measured by RT-qPCR (n = 96). c DKK2 protein levels in 12 paired NSCLC and control samples. d Correlations between DKK2 mRNA and LINC00326 levels in NSCLC samples (n = 96) (r = 0.8754, P < 0.0001). e Correlations between DKK2 mRNA and miR-657 levels in NSCLC tumor samples (n = 96). f Kaplan–Meier curves showing relationship between DKK2 levels and overall survival. g Relative luciferase activity in 293T cells after co-transfection with the luciferase reporter plasmid (carrying either the wt or mut DKK2 -3′-UTR) and either miR-657 mimics or mimics NC. h RIP of DKK2 and miR-657 in A549 and HCC827 cell lysates. Coprecipitated RNA levels were measured by RT-qPCR. i RNA pull-down in A549 and HCC827 cells coprecipitated with biotin-labeled hsa-miR-657. Data are presented as mean ± standard deviation. The experiments were repeated three times. **P < 0.01; ***P < 0.001. ****P < 0.0001