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Fig. 3 | Biology Direct

Fig. 3

From: Upregulation of long intergenic non-coding RNA LINC00326 inhibits non-small cell lung carcinoma progression by blocking Wnt/β-catenin pathway through modulating the miR-657/dickkopf WNT signaling pathway inhibitor 2 axis

Fig. 3

LINC00326 sponges miR-657 in NSCLC cells. a Predicted miR-657-binding sequences in LINC00326 (LINC00326-wt) and the design of the mutant sequence (LINC00326-mut). b miR-657 mRNA levels in NSCLC and control tissues, measured by RT-qPCR (n = 96); c Correlation between LINC00326 and miR-657 levels in tumor samples -n = 96) (r =  − 0. 6105, P < 0.0001). d Association between miR-657 levels and patient overall survival. e miR-657 mRNA levels in HCC827 and A549 cells transfected with OE-CTRL, OE-LINC00326, si-CTRL, or si-LINC00326, measured by RT-qPCR. f Luciferase activity in 293T cells 48 h after transfection with mimics-NC/miR-657 mimic and LINC00326-WT/ LINC00326-MUT. g, h Interaction between LINC00326 and miR-657 shown by RIP and RT-qPCR. i RNA pull-down using biotinylated miR-657. Data are presented as mean ± standard deviation. The experiments were repeated three times. ** P < 0.01; *** P < 0.001. **** P < 0.0001

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Biology Direct

ISSN: 1745-6150

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