Fig. 7

LGG and siSNHG17 improve radiation-induced lung fibrosis. a Male C57BL/6 mice were divided into five groups: control group (Con), radiation-induced lung fibrosis group with 10 Gy radiation treated once time (10 Gy), mice treated with LGG prior to received 10 Gy radiation on lung (10 Gy + LGG), mice treated with siSNHG17 through tail vein injection prior to received 10 Gy radiation on lung (10 Gy + siSNHG17), mice treated with LGG and siSNHG17 prior to received 10 Gy radiation on lung (10 Gy + LGG + siSNHG17). The mice were killed at the 7th weeks to collect tissues for further detection of various parameters. b SNHG17 expression in lung tissues detected by qRT-PCR. c Representative images of hematoxylin and eosin-stained metastatic lung tissues. Scale bar = 50 μm. d Representative photographs of lung tissues by TEM. e Representative blots E-cadherin, and N-cadherin protein expression in lung tissues with or without LGG treatment post 10 Gy radiation by Western blotting assay. f Representative blots NICD, and PTBP1 protein expression in lung tissues with or without LGG treatment post 10 Gy radiation by Western blotting assay. g Representative blots NICD, and PTBP1 protein expression in lung tissues with or without knockdown of SNHG17 post 10 Gy radiation by Western blotting assay. Data are means ± SD (standard deviation). n = 3 independent experiments, Student’s two-tailed unpaired t test was used to compare differences between two groups. *p < 0.05