Fig. 7

Airn/IMP2 stabilized p53 mRNA in an m6A-dependent manner. A, D, K RNA FISH and immunofluorescence co-staining was employed to detect the colocalization of IMP2 protein (red), p53 mRNA (green) and DAPI (blue); Scale bar = 20 μm. B, E, L qRT-PCR analysis of p53 mRNA in CFs. C, F, M The half-life of p53 mRNA in CFs were quantified by qRT-PCR at indicated time points after actinomycin D treatment in CFs. G Top consensus sequence of IMP2-binding sites and the m6A motif and predicted m6A-binding sequences are present in the p53 3’UTR region. H Enrichment of m6A modification in p53 wrote by METTL3 is detected by a gene-specific m6A qPCR assay. I RIP and RT-PCR assays were conducted using anti-IMP2 to verify the binding between IMP2 and p53 mRNA upon silencing METTL3. J Representative blot images and quantitative analysis of p53 upon silencing METTL3. Data are presented as means ± SEM. *p < 0.05; **p < 0.01. n = 3 wells