Fig. 5

LINC01419 recruits EZH2 to regulate FBP1 affecting the properties of LUAD stem cells (CD44⁺). A Western blot was used to detect the protein expression levels of FBP1 in LUAD cells; B CCK-8 was used to detect the proliferation activity of LUAD cells; C Tumor cell sphere-forming assay was used to detect the sphere-forming ability of LUAD cells; D Quantification of the index of the number of tumor spheres in tumor sphere-forming assay; E Western blot was used to detect the expression levels of cell surface markers CD44, CD133, and ALDH-1 in each CSCs treatment group. The cell grouping of A–E was set as follows: sh-NC + sh-NC, sh-LINC01419 + sh-NC, sh-LINC01419 + sh-EZH2 and sh-LINC01419 + sh-FBP1, all of which were transfected into CD44⁺ cells for related studies. *P < 0.05