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Fig. 3 | Biology Direct

Fig. 3

From: LINC00885 promotes cervical cancer progression through sponging miR-3150b-3p and upregulating BAZ2A

Fig. 3

LINC00885 modulated miR-3150b-3p in CC cells. A, B FISH and subcellular fractionation were used to determine the localization of LINC00885 in SiHa and HeLa cells. C RIP assay showed that LINC00885 could be immunoprecipitated in Ago2 antibody. D Based on prediction on starBase and LncBase Predicted v.2, 4 miRNAs (miR-3150b-3p, miR-432-5p, miR-4784, and miR-3613-5p) might bind to LINC00885 (left). RT-qPCR analysis was done for the detection of miR-3150b-3p in different cell lines (right). E RT-qPCR analysis was used to examine the expression of miR-3150b-3p in SiHa cells with LINC00885 overexpression and in HeLa cells with LINC00885 silence. F After prediction of the binding sites between LINC00885 and miR-3150b-3p, luciferase reporter assay was done in HEK293T cells for the evaluation of the interaction between miR-3150b-3p and LINC00885. G, H RIP assay and pulldown assay were used to evaluate the interaction between LINC00885 and miR-3150b-3p. *P < 0.05, **P < 0.01, ***P < 0.001

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