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Fig. 3 | Biology Direct

Fig. 3

From: Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma

Fig. 3

YY1 is the target gene of miR-3614-5p in HCC cells. A The expression of candidate mRNAs was tested through qRT-PCR in miR-3614-5p mimics transfected cells. B The expression of YY1 in HCC cell lines and normal cell line was detected via qRT-PCR. C RIP assay verified the existence of LOXL1-AS1/miR-3614-5p/YY1 in RISC complex. D RNA pull down assay further proved the interaction of miR-3614-5p and YY1. E The binding sites of miR-3614-5p and YY1 were demonstrated. F Luciferase reporter assay proved the combination of miR-3614-5p and YY1 by measuring the luciferase activity of YY1-WT/Mut under the influence of miR-3614-5p mimics transfection. **P < 0.01

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Biology Direct

ISSN: 1745-6150

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