Fig. 2

TFAP2A-AS1 sponges miR-3657 in GC cells. A FISH assay was used to detect the distribution of TFAP2A-AS1 in AGS and NUGC4 cells. B RIP assay was used to detect the enrichment of TFAP2A-AS1 in RISC of AGS and NUGC4 cells. C DIANA database was used to screen out the potential target miRNAs, hsa-miR-876-3p, hsa-miR-4516, hsa-miR-9-5p, hsa-miR-5703, hsa-miR-3131, hsa-miR-4687-3p, hsa-miR-6762-5p, hsa-miR-4434, hsa-miR-3142, hsa-miR-3657 and hsa-miR-1245b-5p. D QPCR was used to detect the knockdown efficiency of sh-TFAP2A-AS1-1/2/3 in AGS cells. E QPCR was used to detect the expression of potential target miRNAs in AGS and NUGC4 cells after the knockdown of TFAP2A-AS1. F RNA-pulldown assay was used to detect the interaction of TFAP2A-AS1 with hsa-miR-4516, hsa-miR-5703, hsa-miR-3131, hsa-miR-4434 or hsa-miR-3657 in AGS and NUGC4 cells. G, H Dual luciferase reporter and RNA-pulldown assays were used to explore the interaction between TFAP2A-AS1 and miR-3657 in AGS and NUGC4 cells. The statistical analysis for B, E was student’s t-test, for D, G was one-way ANOVA, and for F was two-way ANOVA. β-actin was used as the internal reference for gene expression analysis. **P < 0.01