Fig. 5
BMPs was decreased dramatically in the secretory ameloblasts of the Sox2-Cre;Fam20cfl/fl mice. A and B, IHC analyses (signal in brown) of BMP2 on the sagittal sections of the mandibular incisors of 3-day-old Fam20cfl/fl (A) and Sox2-Cre;Fam20cfl/fl mouse (B) a1 and b1 are the higher magnification views of the presecretory ameloblasts in red boxes, a2 and b2 are the secretory ameloblasts in black boxes in A and B, respectively. BMP2 was significantly reduced in the incisors of Sox2-Cre;Fam20cfl/fl mouse compared to the Fam20cfl/fl mouse. C and D, IHC analyses of BMP4 on the sagittal sections of the mandibular incisors of 3-day-old Fam20cfl/fl (C) and Sox2-Cre;Fam20cfl/fl mice (D). c1 and d1 are the higher magnification views of the presecretory ameloblasts in red boxes, c2 and d2 are the secretory ameloblasts in black boxes in C and D, respectively. BMP4 was significantly reduced in the incisors of Sox2-Cre;Fam20cfl/fl mouse compared to the Fam20cfl/fl mouse. E and F, IHC analyses of BMP7 on the sagittal sections of the mandibular incisors of 3-day-old Fam20cfl/fl (E) and Sox2-Cre;Fam20cfl/fl mice (F). e1 and f1 are the higher magnification views of the presecretory ameloblasts in red boxes, e2 and f2 are the secretory ameloblasts in black boxes in E and F, respectively. BMP7 was also reduced significantly in the Sox2-Cre;Fam20cfl/fl mouse incisors, compared to the Fam20cfl/fl mouse incisors. G, Western-immunoblotting analyses of BMP2, BMP4 and BMP7 of the enamel organ cells of 3-day-old Fam20cfl/fl and Sox2-Cre;Fam20cfl/fl mouse incisors. H, Relative protein levels of BMP2, BMP4 and BMP7 determined by Western immunoblotting (n = 3; * = p < 0.05). The protein levels of BMP2, BMP4 and BMP7 were significantly decreased in the enamel organ cells of Sox2-Cre;Fam20cfl/fl mice compared to the Fam20cfl/fl mice. I, Real-time PCR analyses of the mRNA levels of the Bmp2, Bmp4 and Bmp7 in the enamel organ cells of 3-day-old Fam20cfl/fl and Sox2-Cre;Fam20cfl/fl mouse incisors. The mRNA levels of Bmp2, Bmp4 and Bmp7 were not changed in the enamel organ cells of Sox2-Cre;Fam20cfl/fl mice compared to the Fam20cfl/fl mice. Scale bars: 500 μm in A - F; 50 μm in a1- f1; 50 μm in a2 - f2