Fig. 5

Differential essentiality of genes B a between nine DDR-deficient and MCF7 cell lines; and b between PTEN−/− and PTEN wild-type isogenic cell lines. We considered MCF7, which does not have any known DDR defect, as our control. Comparisons of GARP-score means for genes B between DDR-deficient lines and MCF7 showed significant differences (ANOVA p < 0.0001) between these cell lines. Similarly, comparison of GARP scores between two isogenic HCT116-derived cell lines, one with PTEN−/− and the other with wild type PTEN showed significant difference (paired t-test: p < 0.0001) between the two cell lines. This analysis indicated that the essentiality/lethality of genes B is specific to DDR-deficient/PTEN-deficient cell lines, and therefore context-dependent on DDR deficiency