Figure 1

Phylogeny of G. theta Hbs. (A) Bayesian phylogenetic tree of the 13 globin domains of G. theta aligned using MAFFT L-INS-i MSA. Bayesian phylogenetic reconstruction was performed by MrBayes 3.2.2 employing a mixed substitution model. MCMCMC sampling was carried out using 2 independent runs for 1′000′000 generations on the CIPRES web portal [39]. Support values at branches represent Bayesian posterior probabilities (>0.5). (B) Bayesian phylogenetic tree based on a Clustal Omega MSA, of G. theta Hbs with representative vertebrate, protist and choanoflagellate Hbs, using the two Bacillus nonheme globins as outgroup [23]. Bayesian phylogenetic reconstruction was performed by MrBayes 3.2.2 employing a mixed substitution model. MCMCMC sampling was carried out using 2 independent runs for 10′000′000 generations on the CIPRES web portal [39]. All globin sequences are identified by full species name, the number of residues, the abbreviated phylum and family names, and their identification numbers. Support values at branches represent Bayesian posterior probabilities (>0.5). Abbreviations of protist taxons: ALV – Alveolate; AMOE – Amoebozoa; CHOA – Choanoflagellate; CHL – Chlorophyte; FIL – Filasterea; ICH – Ichthyosporea; HAP – Haptophyte; RHO – Rhodophyte; STR – Stramenopile.