Experimental validation of the CvP-bias as a predictor of thermal stability. (A) Thermal denaturation curves for A. pompejana (Apo), human (Hum), Saccharomyces cerevisiae (Sce) and Schisosaccharomyces pombe (Spo) recombinant Rrp4 proteins. (B) Correlation between CvP-bias difference and Tm difference in orthologous protein pairs with experimentally determined Tm values from A. pompejana and C. thermophilum. Shading indicates the areas where the values are expected, if the predictors work. Related to Table 4.