LA-loaded moDC induced Th1 cytokine production of γδ T cells, predominantly TCRVδ2+ T cells. (A) Purified γδ T cells were incubated for 48 h with or without LA, in the presence or absence of moDCs, plus or not plus blocking antibody. For a determination of the cytokine secretion, supernatants were collected and cytokine concentration was measured by CBA kit. The results represent mean ± SD of three independent experiments. (B) γδ T-enriched PBMCs are stimulated by LA-loaded moDC for 2 weeks, then collected and double stained as described in Materials and Methods using human FITC-TCRVδ2/PE-IFN-γ (Left), and FITC-TCRVδ 1/PE-IFN-γ (right). One of two similar experiments is shown.