Proportion of pluripotent cells among maGSC Stra8 SSC5 cells cultured under various conditions. (A) MaGSC Stra8 SSC5 cells were cultured in basic medium (condition I), medium plus LIF (condition II), basic medium on MEFs (condition III), in medium plus LIF on MEFs (condition IV). ESCs (line ESC 129Sv MPI-II) were cultured under condition IV. The proportion of SSEA-1 and Oct-3/4-positive cells was evaluated by flow cytometry. The means of EGFP-positive cells + standard deviation (SD) are shown (n = 3). (B) MaGSC Stra8 SSC5 cells cultured under conditions I to IV or cells differentiated in vitro for 5 plus 10 days (diff.) were analyzed for EGFP expression by flow cytometry. Representative histograms are shown. An EGFP-negative ESC line (ESC 129Sv MPI-II) is included as negative control. (C) The means of EGFP-positive cells + SD are shown (n = 5).