Figure 1

EF-2 contains a derived insert. A) Structural alignment of EF-G from Thermus thermophilus (2BV3 61–89 colored blue), EF-Tu (1EFC 58–86 colored cyan) from Escherichia coli, EF-2 from Saccharomyces cerevisiae (1N0U 67–110 colored red). B) Sequence corresponding to the structural alignment in A. The well conserved glycine and aspartic acid are highlighted green and magenta respectively in both the sequence and structure to show the disordered nature of this region. The 4 positions highlighted in red are aligned in the original alignment, which is why the alignment was critiqued in [22]. The additional insert in Eukaryotes relative to the Archaea is boxed in black.