Fig. 7

XIST promoted proliferation, fibrosis, and autophagy through regulating the XIST/miR-302b-3p axis in HLF cells. A The expression levels of miR-302b-3p in HLF cells infected with the indicated adenovirus were evaluated by RT-qPCR. B Growth curves of HLF cells infected with theindicated adenovirus were evaluated by CCK-8 assays. C and D The apoptosis ratios of HLF cells infected with the indicated adenovirus were assessed by flow cytometry with Annexin V/PI double staining. E and F The expression of apoptosis-related proteins (cleaved caspase3, Bax, and Bcl-2) in HLF cells infected with the indicated adenovirus was measured by western blotting. E and H The expression of fibrosis-related proteins (collagen I, collagen III, MMP2, and MMP13) in HLF cells infected with the indicated adenovirus was confirmed by western blotting. G and I The expression of autophagy-related proteins (LC3B, Beclin1, p62, p-AKT, Akt, p-mTOR, and mTOR) in HLF cells infected with the indicated adenovirus was confirmed by western blotting. C-casp3, cleaved-caspase-3; OE-NC, corresponding negative control of OE-XIST; OE-XIST, the overexpression adenovirus of XIST; OE-miR-NC, corresponding negative control of OE-miR-302b-3p; OE-miR-302b-3p, the overexpression adenovirus of miR-302b-3p. ^*P < 0.05, ^**P < 0.01, and.^***P < 0.001