Fig. 6

MiR-302b-3p inhibited proliferation, fibrosis, and autophagy through mediating the miR-302b-3p/VEGFA axis in HLF cells. A The VEGFA expression in HLF cells infected with the indicated adenovirus was evaluated by RT-qPCR. B Growth curves of HLF cells infected with the indicated adenovirus were evaluated by CCK-8 assays. C and D The apoptosis ratios of HLF cells infected with the indicated adenovirus were assessed by flow cytometry with Annexin V/PI double staining. E and F The expression of apoptosis-related proteins (cleaved caspase3, Bax, and Bcl-2) in HLF cells infected with indicated adenovirus was measured by western blotting. E and H The expression of fibrosis-related proteins (collagen I, collagen III, MMP2, and MMP13) in HLF cells infected with the indicated adenovirus was confirmed by western blotting. G and I The protein expression of autophagy-related proteins (LC3B, Beclin1, p62, p-AKT, Akt, p-mTOR, and mTOR) in HLF cells infected with the indicated adenovirus was confirmed by western blotting. C-casp3, cleaved-caspase-3; shNC, corresponding negative control of shVEGFA; shVEGFA, the RNAi adenovirus against VEGFA; OE-NC, corresponding negative control of OE-VEGFA; OE-VEGFA, the overexpression adenovirus of VEGFA; OE-miR-NC, corresponding negative control of OE-miR-302b-3p; OE-miR-302b-3p, the overexpression adenovirus of miR-302b-3p; sh-miR-NC, corresponding negative control of sh-miR-302b-3p; sh-miR-302b-3p, the RNAi adenovirus against miR-302b-3p. ^*P < 0.05, ^**P < 0.01, and.^***P < 0.001