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Fig. 3 | Biology Direct

Fig. 3

From: LncRNA XIST facilitates hypertrophy of ligamentum flavum by activating VEGFA-mediated autophagy through sponging miR-302b-3p

Fig. 3

XIST knockdown inhibited LF hyperplastic fibrosis in vivo. A XIST expression in LF tissue from different treated mice was determined by RT-qPCR. B Representative images of H&E staining of the LF and quantitative analysis of the LF area. Scale bar = 100 μm. Representative images of EVG staining of the LF and quantitative analysis of elastic fiber area to collagen fiber area. Scale bar = 20 μm. C and D The expression of apoptosis-related proteins (cleaved caspase3, Bax, and Bcl-2) in LF tissues from different treated mice were measured by western blotting. C and E The expression of fibrosis-related proteins (collagen I, collagen III, MMP2, and MMP13) in LF tissues from different treated mice were evaluated by western blotting. Ctrl, control mice; HLF, HLF mice induced by bipedal standing; AAV-shNC, corresponding negative control of AAV-shTCF7; AAV-shTCF7, the RNAi adeno associated virus against XIST. *P < 0.05, **P < 0.01, and.***P < 0.001

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