Fig. 3From: Inhibition of TRAF6 improves hyperlipidemic acute pancreatitis by alleviating pyroptosis in vitro and in vivo rat modelsInhibition of TRAF6 suppressed pyroptosis in AP rat models. (A) Ultrastructure of pancreatic acinar cells from AP rats was observed under transmission electron microscope; N: nuclear, M: mitochondrion, Z: zymogen granules, ER: Endoplasmic reticulum. (B) The expression of GSDMD in the pancreatic tissue of AP rats was detected by immunofluorescent staining (400×). (C) Pyroptotic cells in the pancreatic tissue of AP rats were detected by TUNEL staining (200×). D, E) The levels of TLR9, TRAF6, NLRP3, and pyroptosis-relaxed proteins in the pancreatic tissue of AP rats were determined by RT-qPCR and Western blot. F) The expression of GSDMD in pancreatic tissue of AP rats was detected by immunohistochemistry staining (200×). *P < 0.05, **P < 0.01 and ***P < 0.001Back to article page