Fig. 3
From: Probing pathways by which rhynchophylline modifies sleep using spatial transcriptomics
Target protein levels are generally not modified by RHY but correlated with sleep variables
(A) Protein levels in total and synaptoneurosomal (Syn) fractions were quantified for the cerebral cortex, hippocampus, and a thalamus/hypothalamus spanning region. Except for the pGLUR1/GLUR1 ratio of the total protein fraction in the male hippocampus (F2,11 = 4.7, p = 0.03), no significant effect of treatment was found (see Fig. S2A for one way ANOVA results; n = 3–11 per group). The blue diamond at the top of a graph indicates a significant difference between RHY100 and RHY50 in males (post hoc comparison p < 0.05).
(B) Correlations (including both female and male data) between protein levels and sleep variables showed significant correlations between cerebral cortex GLUR1 level (total fraction) and time spent in SWS or PS between ZT13 and ZT17; and between thalamic/hypothalamic GLUR1 (Syn fraction) and time spent in PS between ZT13 and ZT17.