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Fig. 5 | Biology Direct

Fig. 5

From: TM4SF1 upregulates MYH9 to activate the NOTCH pathway to promote cancer stemness and lenvatinib resistance in HCC

Fig. 5

TM4SF1 could activate the NOTCH pathway through the upregulation of MYH9. A Genes whose expression changed after silencing TM4SF1 were screened via protein mass spectrometry, and the genes related to the NOTCH pathway and cancer stemness were gradually screened out via the correlation analysis of the GEPIA database. B Correlation of MYH9 with cancer stem–related molecules and the NOTCH pathway–related molecules via the correlation analysis of the GEPIA databases. C Using the TCGA database, the association between the expressions of TM4SF1 and MYH9 in 371 patients with HCC was examined. D Results of the Co-IP experiment showed that TM4SF1 could pull down MYH9. E WB analysis showed that the expression of MYH9 was down-regulated after TM4SF1 silence, but the expression of TM4SF1 was not affected after MYH9 silence. F Results of the qPCR analysis verified the expression efficiency after silencing MYH9. G Results of the WB analysis verified the expression efficiency after silencing MYH9. H The results of the qPCR test revealed that the expression level of molecules connected to the NOTCH pathway dropped when MYH9 was silenced. I The results of the WB analysis demonstrated that silencing MYH9 reduced the expression of molecules involved in the NOTCH pathway

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