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Fig. 4 | Biology Direct

Fig. 4

From: TM4SF1 upregulates MYH9 to activate the NOTCH pathway to promote cancer stemness and lenvatinib resistance in HCC

Fig. 4

TM4SF1 could activate the NOTCH pathway to promote cancer stemness. A The cancer stem–related NOTCH pathway was obtained via the enrichment of the TM4SF1-related pathways using the GSEA technology. B The qPCR analysis revealed that after TM4SF1 was silenced, the expression of molecules connected to the NOTCH pathway reduced. C The WB analysis revealed that after TM4SF1 was silenced, the expression of molecules connected to the NOTCH pathway reduced. D Primary grown tumor cells from nude mice were used to harvest RNA. Following the silencing of TM4SF1, the qPCR analysis revealed the expression of molecules connected to the NOTCH pathway. E The results of the WB analysis demonstrated that, following TM4SF1 silencing, primary tumor cells from nude mice expressed chemicals relevant to the NOTCH pathway. F WB analysis showed that overexpression of TM4SF1 could reverse the inhibitory effect of the NOTCH pathway targeted inhibitor RO4929097 on the molecules connected to the NOTCH pathway. *P < 0.05; **P < 0.01; ***P < 0.001

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