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Fig. 3 | Biology Direct

Fig. 3

From: TM4SF1 upregulates MYH9 to activate the NOTCH pathway to promote cancer stemness and lenvatinib resistance in HCC

Fig. 3

Silencing TM4SF1 could inhibit the growth and stemness in HCC in vivo. A Subcutaneous tumor removed from nude mice after 45 days of culture. B Statistical comparison of tumor volume between knockdown TM4SF1 and control groups. C Statistical comparison of tumor weight between knockdown TM4SF1 and control groups. D Immunohistochemical results of subcutaneous tumors in nude mice showed that after silencing TM4SF1, the expression of cancer stem–related molecules decreased with the decrease in TM4SF1 expression. Scale bars, 30 ×: 300 μm, 100 ×: 100 μm. E RNA was extracted from primary cultured tumor cells of nude mice. The qPCR analysis showed that the expression of cancer stem–related molecules decreased after silencing TM4SF1. F Results of the WB analysis showed that after TM4SF1 silencing, the expression of cancer stem–related molecule decreased in primary tumor cells of nude mice. *P < 0.05; **P < 0.01; ***P < 0.001

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Biology Direct

ISSN: 1745-6150

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