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Fig. 3 | Biology Direct

Fig. 3

From: IGF2BP2 promotes colorectal cancer progression by upregulating the expression of TFRC and enhancing iron metabolism

Fig. 3

IGF2BP2 promotes iron metabolism in colorectal cancer. (A) Gene Ontology (GO) analysis of the 20 relevant pathways in HCT-116 cells enriched using RNA-seq. (B) Intracellular iron levels were measured using an iron assay kit after transfecting HCT-116 and SW480 cell lines with IGF2BP2 siRNA. (C) Calcein-AM (C-AM) was used to quantify cellular LIP levels via spectrophotometric measurements at an absorbance wavelength of 525 nm. Subtract the mean fluorescence intensity (MFI) of C-AM from the MFI of C-AM treated with DFO. (D) ROS content in HCT-116 and SW480 cells were measured after IGF2BP2 knockdown. Each value represents the mean ± SD for triplicate samples (Student’s t-test). **P < 0.01, ***P < 0.001 and **** P < 0.0001

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Biology Direct

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