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Fig. 2 | Biology Direct

Fig. 2

From: IGF2BP2 promotes colorectal cancer progression by upregulating the expression of TFRC and enhancing iron metabolism

Fig. 2

Downregulation of IGF2BP2 inhibits the proliferation and cell cycling of colorectal cancer cell lines. (A, B) HCT-116 and SW480 cells were transfected with IGF2BP2 siRNA, and then IGF2BP2 mRNA (A) and protein (B) levels were determined by qPCR and western blotting, respectively. (C-E) After IGF2BP2 silencing, the viability (C), colony forming ability (D), and proliferative ability (EdU assay) (E) of the cells were evaluated. Scale bars: 150 μm. (F, G, H) The effect of IGF2BP2 on the cell cycle. (F), HCT-116 and SW480 cells were transfected with the IGF2BP2 siRNA and subjected to flow cytometry after propidium iodide (PI) staining. After transfection, the cell cycle-related gene expression and protein levels were assessed using qRT-PCR (G) and western blotting (H), respectively. Each value represents the mean ± SD for triplicate samples (Student’s t-test). *P < 0.05, **P < 0.01, ***P < 0.001 and **** P < 0.0001

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