Fig. 2From: IGF2BP2 promotes colorectal cancer progression by upregulating the expression of TFRC and enhancing iron metabolismDownregulation of IGF2BP2 inhibits the proliferation and cell cycling of colorectal cancer cell lines. (A, B) HCT-116 and SW480 cells were transfected with IGF2BP2 siRNA, and then IGF2BP2 mRNA (A) and protein (B) levels were determined by qPCR and western blotting, respectively. (C-E) After IGF2BP2 silencing, the viability (C), colony forming ability (D), and proliferative ability (EdU assay) (E) of the cells were evaluated. Scale bars: 150 μm. (F, G, H) The effect of IGF2BP2 on the cell cycle. (F), HCT-116 and SW480 cells were transfected with the IGF2BP2 siRNA and subjected to flow cytometry after propidium iodide (PI) staining. After transfection, the cell cycle-related gene expression and protein levels were assessed using qRT-PCR (G) and western blotting (H), respectively. Each value represents the mean ± SD for triplicate samples (Student’s t-test). *P < 0.05, **P < 0.01, ***P < 0.001 and **** P < 0.0001Back to article page