Fig. 3

Knockdown of RP11-620J15.3 inhibits glycolysis in HCC. A Positive enrichment for the gene signature associated with fatty acid metabolism between the low and high RP11-620J15.3 expression groups of the RNA-seq dataset. B Nontargeted metabolomics GC–MS analysis of glycolysis and TCA cycle metabolites in HCC cells. C Heatmap displaying changes in the metabolites of glycolysis or OXPHOS. D–F Changes in the glycolysis-related metabolites of HCC cells. G Changes in the ECAR rate of HCC cells. H, I In the HCC cells with RP11-620J15.3 knockdown, the mRNA and protein levels of the related lipogenic enzymes were significantly reduced. J, K Pearson’s correlational analysis of RP11-620J15.3 and GPI in the TCGA-LIHC dataset and 80 pairs of tissues from Zhengzhou University. *p < 0.05, **p < 0.01, ***p < 0.001