Fig. 3

SNHG17 can interact with PTBP1 post radiation. a A549 cells were divided into 2 groups, knockdown of SNHG17 group (siSNHG17), treated with IR followed by knockdown of SNHG17 group (siSNHG17-IR). Cells were collected after treatment and send to BiotechPack Seientific Ltd. Beijing, China for LC–MS/MS detection to discover potential SNHG17-interacted proteins. b GO assay of the potential biological function for differential binding proteins. c KEGG assay of the potential biological pathway for differential binding proteins. d Quantitative analysis of PTBP1 mRNA expression in knockdown and overexpression of SNHG17 in A549 cells by qRT-PCR assay. e Representative blots of PTBP1 protein expression in knockdown of SNHG17 in A549 cells post 6 Gy radiation by Western blotting assay. f Representative blots of PTBP1 protein expression in overexpression of SNHG17 in A549 cells post 6 Gy radiation by Western blotting assay. g RNA pull-down analysis of the binding of SNHG17 with PTBP1 in total protein extracted from A549 cells post radiation. Data are means ± SD (standard deviation). n = 3 independent experiments, Student’s two-tailed unpaired t test was used to compare differences between two groups. *p < 0.05