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Fig. 5 | Biology Direct

Fig. 5

From: LINC00858 stabilizes RAN expression and promotes metastasis of gastric cancer

Fig. 5

The transcription factor YY1 upregulates LINC00858 expression. A Genomic alteration of LINC00858 in GC was analyzed from the cBioPortal dataset. B The transcription factor YY1 binding motif was predicted by bioinformatic analysis. A scheme of the LINC00858 promoter with a YY1 binding site downstream of the TSS is shown. C Correlations between YY1 and LINC00858 in the TCGA-STAD database. D, E Western blotting of YY1 protein (D) and qPCR of LINC00858 (E) expression in HGC27 and MKN74 cells transfected with siRNAs. F ChIP-qPCR assay indicating the direct binding of YY1 to the LINC00858 promoter region in HGC27 and MKN74 cells. G Electrophoresis of the qPCR products indicates the direct binding of YY1 to the LINC00858 promoter region in HGC27 and MKN74 cells. H YY1 expression in paired and unpaired TCGA-STAD tumors and adjacent normal tissues. I Kaplan–Meier analysis of the correlation between YY1 expression and overall survival (left) and disease specific survival (right) in the TCGA-STAD database. J Representative images of immunohistochemical analysis of YY1 in LINC00858 low- and high-expressing samples (left panel). Associations between LINC00858 expression and protein level of YY1 in GC tissues (right panel). K Representative IHC images of YY1 in the adjacent normal tissues, GC, lymph nodes and distant metastasis. Scale bars = 100 μm (left panel). IHC scores of YY1 expression were calculated in the right panel. L Western blotting detection of YY1 expression indicates higher expression in tumor tissues than paired adjacent normal tissues in 10 patients from SYSUCC. The P values in E, F, H and K were calculated using a two-sided paired and unpaired Student’s t test. The P value in J (right) was calculated using chi-square test *P < 0.05

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