Skip to main content
Fig. 4 | Biology Direct

Fig. 4

From: LINC00858 stabilizes RAN expression and promotes metastasis of gastric cancer

Fig. 4

LINC00858 protects RAN from ubiquitination and proteasomal degradation in GC. A qPCR detection of RAN expression in HGC27 and MKN74 cells upon knockdown of LINC00858. B Western blotting of RAN expression in HGC27 and MKN74 cells upon knockdown of LINC00858. C Correlations between RAN and LINC00858 in the TCGA-STAD database. D Downregulation of RAN caused by LINC00858 knockdown was abolished by MG-132(10 μM, 12 h) in HGC27 and MKN74 cells. E IP assays showed that LINC00858 knockdown resulted in increased ubiquitination of RAN in HGC27 and MKN74 cells. F HCG27 and MKN74 cells with or without shRNAs specific for LINC00858 were treated with 20 μg/ml CHX for the indicated periods of time. RAN levels were analyzed by western blotting. G The effects of LINC00858 knockdown on migration and invasion were reversed by overexpression of RAN in HGC27 and primary GC cells. Representative images (left panel) and quantification data (right panel) are shown. The P values in A and G were calculated using two-sided unpaired Student’s t test. *P < 0.05 and N.S. indicate nonsignificant

Back to article page