Fig. 2

LINC00858 is overexpressed in multiple GC cell lines and promotes cell migration and invasion. A Representative images of migration and invasion analysis in HGC27 cells upon knockdown of LINC00858. B Quantification of migrated and invaded HGC27 cells upon knockdown of LINC00858. C Representative images of migrated MGC803 and BGC823 cells upon overexpression of LINC00858. D Representative luciferase imaging of lung metastatic cells in nude mice after LINC00858 knockdown. E Representative results of hematoxylin and eosin (HE) staining (left) of metastatic lung nodules from mice injected with LINC00858 knockdown and control HGC27 cells via the tail vein. Number of mice with or without lung metastases were analyzed (Upper right). Metastatic nodules under microscope were counted and recorded (Lower right). F Schematic diagram illustrating the generation of orthotopic xenografted models. G Representative liver metastasis of HGC27 cells inoculated orthotopically were photographed and stained with HE. The number of mice with or without liver metastasis was documented. H Representative metastatic lymph nodes of HGC27 cells inoculated orthotopically were photographed, and the number of mice with or without lymph node metastasis was documented. The P values in B and E (lower) were calculated using two-sided unpaired Student’s t test, E (upper) and those in G and H were calculated using chi-square test. *P < 0.05