Fig. 1

LINC00858 is overexpressed in TCGA-STAD database and is associated with poor clinical outcome. A Flowchart of this study. B Heatmap of the top 20 candidate lncRNAs that were highly expressed between STAD and normal gastric tissues and significantly associated with OS in the TCGA database. C Correlations between the 20 lncRNAs with prognosis in STAD according to the TCGA database. The column represents P value of each lncRNA and the dotted line represents P = 0.05. The solid line represents the HR (Hazards Ratio) value. D qPCR detection of LINC00858 expression in 55 paired tumor and normal gastric tissues. E qPCR detection of LINC00858 expression in 100 paired tumor and normal colorectal tissues. Data in D and E are shown as the mean ± SD. △CT on Y-axis indicates the Ct value of the internal control subtracted from that of the target genes, and lower values indicate higher expression. F LINC00858 expression in TCGA tumors and normal tissues with the data of the GTEx database as controls. G Kaplan‒Meier analysis of the correlation between LINC00858 expression and overall survival and disease free survival in the SYSUCC cohort. H Kaplan–Meier analysis of the correlation between LINC00858 expression and disease specific survival in the TCGA-STAD database. The P values in D-F were calculated using a two-sided paired or unpaired Student’s t test. *P < 0.05